Implications of Chronic Exposure of Petrolchemicals on Mood Like Regulatory Neurochemical Profile and Brain Specific Histo Architectural Integrity in Swiss Adult Male Mice.

Background: Chronic exposure to various chemicals in the environment has been a growing concern due to their potential impacts on human health. One such product of interest is Petrol and gas which comes from crude oil and is a mixture of organic hydrocarbons like toluene and benzene. The present study investigates the implications of chronic exposure to petrolchemicals on mood-regulating neurochemical profiles and histo architectural integrity in swiss adult male mice. Understanding the potential effects of Petrolchemicals on mental health can help inform regulatory guidelines and promote public health awareness. Furthermore, when these chemicals are taken into the body, they readily bioaccumulate, thus bind to the brain and fatty tissues. These chemicals are known to induce or cause diverse kinds of neurological disease conditions such as aggression, stress, anxiety, depression and motor disorders. Aim of study : to ascertain the implications of chronic exposure of petrolchemicals on depression mood like regulatory neurochemical profile and brain specific histo architectural integrity in swiss adult male mice. Method: The petrol was obtained from a local filling station and Gas was obtained from a local gas station both located in Abraka, Delta state. Subjects(Swiss adult male mice) were randomly selected into 4 groups of 8 subjects in each exposure cages (compartment). Subjects were exposed to the petrol and gas in their compartment for a period of 4 hours daily for 36 days, after which subjects were returned to their holding facilities.The animals were removed from their cages respectively and place inside separate cages. While some were exposed to petrol only, some were exposed to gas only and some were exposed to both patrol and gas. For the petrol, a small cotton wool was soaked inside the petrol and placed inside a small whitish container with punctured hole that was well covered and placed inside the separate cage, it was allowed to remain inside for 10mins, after 10mins the whitish container was removed and the mice were allowed to rest for 20-30mins before repeating the same procedure for 4hours. For the gas, gas hose was placed (connected to the gas) inside the separate cage and on for 3 seconds, after three seconds,you off the cylinder, after 10mins of exposure to the gas,the mice were allowed to read for 30mins before repeating the procedure. Subjects were randomly selected into 4 groups of 8 subjects in each exposure cages (compartment) out of the 8 mice in each group,6 were used for behavioral study and 2 were used for histological study. After an overnight fast on the last day of the experiment, the animals were sacrifice by cervical region dislocation (decapitation), then the brain tissue was harvested and fixed in 10% formasaline solution in universal containers and were transported to the histology laboratory. Results: The investigation into the implications of chronic exposure to petrol and gas on male Swiss mice using monoamine oxidase (MAO) profile, dentate gyrus,brain stem and amygdala has yielded significant insights into potential biochemical and histological effects. Determination of monoamine oxidase, dentate gyrus, substantial Nigra and amygdala level when exposed to Petrol and gas showed significant decrease when compared to the control group.