Biochemical Effects of Datura Stramonium on Cadmium-Induced Cerebellar Neurotoxicity in Adult Male Wistar Rats

Cadmium is a toxic metal, which poses significant health risks, cancers and neurodegenerative diseases. Datura stramonium known for its diverse medicinal properties, contains biologically active substances that may counteract cadmium toxicity. The aim of this project is to investigate the biochemical effects of Datura Stramonium aqueous leaf extract (DSALE) on cadmium-induced toxicity in the cerebellum of adult male Wistar rats. The study includes the assessment of the level of oxidative stress biomarkers in the cerebellum of adult male Wistar rats - Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (CAT). Twenty-five Wistar rats (180–230g) were randomly divided into five groups (n=5). Group 1 received distilled water (1ml). Group 2 was injected intraperitoneally with cadmium (30mg/kg) on day 1. Group 3 received cadmium (30mg/kg) on day 1 and a DSALE (200mg/kg) orally for 28 days. Group 4 received cadmium (30mg/kg) on day 1 and a lower dose of the DSALE (100mg/kg) orally for 28 days. Group 5 was given only the DSALE (200mg/kg) orally for 28 days. The aim of this project research is to investigate the biochemical effects of Datura Stramonium on cadmium induced toxicity in the cerebellum of adult male wistar rats. Thirty rats weighing between 180-230g were grouped randomly into six groups, each group having five rats. The rats were induced with cadmium intraperitoneally on day 1 and the extract was administered orally for 28 days. In conclusion, based on these findings, this study shows cadmium’s toxicity that causes an imbalance between antioxidant and oxidants in cerebellar tissues, depleting defenses against reactive oxygen species (ROS) and making the tissues more vulnerable. The study indicates when Datura Stramonium is administered in controlled doses, it possesses antioxidant properties that can mitigate oxidative stress and damage in the cerebellum of rats.