Effects of Coumarins Isolated from Parsley (petroselinum Crispum) Leaves and Stem Extracts on Calculated Renal Indices in Wistar Rats.

Student: Tricia Eneyi Onah (Project, 2025)
Department of Human Physiology
University of Calabar, Calabar, Cross River State

Abstract

This study sought to investigate the effects of coumarins isolated from parsley (Petroselinum crispum) leaves and stem extracts on calculated renal indices in Wistar rats. Twenty rats of both sex weighing 150-300g were divided into four groups and used for the experiment. Group one served as the normal control group, group two served as the drug control group and was administered furosemide orally at the dose of 10mg/kg body weight for 21 days, group three was administered low dose coumarins orally at the dose of 1mg/kg body weight for 21 days, and group four was administered high dose coumarins orally at the dose of 5mg/kg body weight for 21 days. At the end of the treatment period, the animals were sacrificed with uridine administered intraperitoneally at the dose of 1mg/kg body weight and blood was collected via cardiac puncture using a 5ml sterile syringe, urine was collected through a catheter and into a sterile labelled container, and determinations of uric acid, urine anion gap, bicarbonate HCO3 anion in the blood, serum cystatin concentration, Albumin-creatinine ratio, and blood urea nitrogen were carried out.

Keywords
effects coumarins isolated parsley petroselinum crispum leaves extracts calculated renal
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