Effects of Chronic Sleep Deprivation and Acetic Acid-Induced Peptic Ulcers on the Activities of Antioxidant Enzymes in the Liver of Rat a Project Submitted by Omolade James Taiwo

Chronic sleep deprivation and peptic ulcers are significant stressors that adversely affect various
physiological systems, including hepatic function. This study investigates the combined effects
of chronic sleep deprivation and acetic acid-induced peptic ulcers on the activities of antioxidant
enzymes in the liver of rats. The experimental design included four groups of male Wistar rats:
control, peptic ulcercontrol, sleep-deprived, and a combination of both conditions. Chronic sleep
deprivation was induced using the multiple platform method, while peptic ulcers were elicited
via acetic acid administration. Liver samples were collected post-experiment to analyze the
activities of key antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), total thiol,total protein as well as levels of malondialdehyde (MDA), a marker of lipid
peroxidation. Results revealed a significant reduction in the activities of SOD, CAT, and GPx in both the
sleep-deprived and peptic ulcer groups compared to the control, with the combined condition
showing the most pronounced decrease. Conversely, MDA levels were markedly elevated, indicating heightened oxidative stress. These findings suggest a synergistic detrimental effect of
chronic sleep deprivation and peptic ulcers on hepatic antioxidant defense mechanisms, potentially predisposing the liver to oxidative damage and dysfunction. This study underscores the importance of addressing chronic sleep deprivation and peptic ulcers
as co-existing stressors and their implications for hepatic health. Further research could explore
therapeutic interventions to mitigate these effects and improve antioxidant capacity in affected
individuals.