Chromatographic Analysis of Secondary Metabolites from Actinomycetes

Chromatography is a powerful analytical technique widely utilized for the separation and characterization of extracts or broth. This study explores the application of chromatography in analyzing secondary metabolites extracted from actinomycete strains. The actinomycetes were grown in Starch Casein broth for 10 days at 280C. After incubation, the broths of the organisms were subjected to cold centrifugation at 4000rpm for 30 mins. The centrifugation was repeated on the filtrate to obtain good extract. Gas Chromatography-Mass Spectrometry technique was used to analyze the extract. Isolates SE21 had a total of 23 different compound in it while SB31 had a total of 18 different compounds. In the results, Isolates SE21 had a total of 23 different compound in it while SB31 had a total of 18 different compounds. GC-MS chromatographic analysis of metabolites from Actinomycetes Isolates SB31, showed compounds such as, propanediol, furanone, dihydro-5-methyl, formic acid, butyl ester, p-xylene, phthalon, di-2-aminobutyric acid, benzoic ethydroxy-acid, phenylethyl alcohol, 3-hexanone, 2,2-dimethyl, L-pyroglutamic acid, Dodecane 2,6,11-trimethyl, methyl stearate, oleic acid, 13-docosenoic acid, Eicosanoic acid, methyl ester, tetracosanoic acid, methyl ester. While the compound detected from Comparative GC-MS Analysis of Metabolites from Actinomycetes Isolates E21 are Benzoic acid, 4-hydroxy-3-5-dimethoxy, glucose, Dodecanoic acid, 9,12-Octadecadienoic acid (Z,Z)-, 9-Octadecenoic acid, methyl ester, 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-, n-Hexadecanoic acid, Octyl beta-D-glucopyranos, Methyl stearate, Oleic Acid, 13-docosenoic acid, Melibiose, Tetracosanoic acid, methyl ester. Isolate SB31 and SE21 were found to have similar compounds exhibiting different retention time and compound weight. Isolate SE21 was found to produce unique compounds such as Phenol, 2-methoxy-4-propyl-, Nonanoic acid, Dodecane 2,6,11-trimethyl and Eicosanoic acid. suggesting potential antimicrobial and antioxidant properties. Several peaks of each of the compound analysed in the compound was also observed. This study demonstrates how integrating chromatography techniques enhances our understanding of the metabolic capabilities of actinomycetes, paving the way for biotechnological applications and the development of novel therapeutics.
Keywords: Secondary Metabolites, Actinomycetes, Gas chromatography-mass spectrometry, Bioactive Compound