Effect of Vitamin C Pretreatment in Cigarette Smoke Induced Oxidative Stress Test

Cigarette smoke is a complex mixture of toxic chemicals and carcinogens that can have profound and detrimental effects on human health. Inhalation of cigarette smoke leads to exposure to numerous harmful substances, including nicotine, tar, carbon monoxide, and various toxic gases. Cigarette smoking contributes to cancer, and other respiratory disorders. Cigarette smoke has systemic effects, increasing the risk of cardiovascular diseases, cancer and adverse reproductive outcomes. This research however sought to determine Effect of Vitamin C pretreatment in cigarette smoke induced oxidative stress. After acclimatization, the rats were randomly allocated to three experimental groups,(n = 6 per group) Group 1 served as the control and was supplied clean water throughout theexperiment. Group 2 served as the cigarette exposed group. Group 3 served as the cigarette exposedand 1000mg/kgbwt {kgbwt = Kilogram bodyweight} vitamin C treatment group. At the end of theexperimental study period (21 days), the animals were cervically decapitated and the blood sample was obtained from the animal and kept in EDTA bottles. The blood samples were centrifuged and the serum was separated and various hematological parameters were analyzed following standard laboratory techniques. From this study, Cigarette alone increased the PCV, Hgb and RBC though insignificantly (p