Bacterial Analysis of Urine Polluted Environment in Benue State University, Makurdi

AbstractThe dissolved substances in the urine can be utilized by microorganisms of various groups as nutrients whenever urine finds its way into the environment. The aim of this study was to isolate and identify Bacteria associated with urine polluted environment (soil). Fifty (50) samples were collected from the urine soil polluted sites (30) samples from first campus and 20 samples from second campus) and control soil site was collected from the topsoil (between 5-20cm), respectively. The samples from polluted soils and control area were collected in separate sterile polythene bags, and were sealed and appropriately labeled and were taken to the Laboratory for immediate bacteriological analysis. The microbial loads of the Samples were determined using the Pour plate method of inoculation. the media used in this work (Nutrient agar, Manitol Salt agar, Mackoncay agar, Xylose-Lysine Deoxycholate agar, Eosin Methylene Blue agar, Peptone Water, Simmon’s Citrate agar, Cystine Lactose Electrolyte-deficiency agar, Triple Sugar Iron agar, Urea Agar Base and Sulphide Indole Motility Media) were prepared according to their various manufacturer’s instructions. The samples were inoculated onto Manitol Salt agar, Mackoncay agar, Xylose-Lysine Deoxycholate agar, Eosin Methylene Blue agar and incubated for 24 hrs. The colonies obtained were further sub-culture to obtain a pure isolate. The isolates were identified using Morphological and Biochemical tests. The isolates obtained were Klebsiella spp., S. aureus, Escherichia coli, Salmonella spp. and Bacillus spp. Bacillus spp. had the highest occurrence rate of 25(39.7%). The prevalence of the isolates were statistically significant (P