Investigation of the Potential of Vinegar Stem in Bioremediation of Petroleum Hydrocarbon Polluted Sandy Soil

The research on the performance evaluation of bio stimulant in crude oil remediation using vinegar stem (Sun dried and Room dried) was experimented on. The physio chemical parameters of the bio stimulant (vinegar stem), Sandy soil sample and the crude oil samples was determined using experimental concepts and the parameters measured are pH , electrical conductivity (EC), total organic carbon (TOC), Total nitrogen (T.N), Total phosphorus (T.P), and potassium. The microbial isolation and identification from the crude oil and the soil sample was carried out and pseudonomas sp and mucus was the most populated organisms in the crude oil and the Sandy soil and the two organisms identified are bacteria (pseudonomas sp) and fungi (mucus), which was cultured and introduced into each bioreactor set. The degradation of the Total petroleum Hydrocarbon (TPH) and the microbial concentration was monitored closely with respect to time. The results obtained after 42 days sampling were used in the determination of the specific rate of TPH degradation in each of the bioreactor set up. The percentage removal of the TPH increases with increase in contact time. This shows that the process was not inhibited by any of the physio chemical properties of controlling the system. Furthermore, the maximum specific rate of the TPH degradation and the equilibrium or dissociation constant of TPH degradation was determined by applying the concept of Lineweaver Burks plot model, Hanes Plot model and Eadic Hofstee plot model all derived from Michaelis Menten model. The R max ranges from 10000 to 20000 for sun dried and R max ranges from 20000 to 20000 for Room dried. However the bio stimulatant performance evaluation in terms of percentage removal of TPH using vinegar stem as bio stimulant revealed 99.1453% for ropm dried sample and 98.317% For sun dried as when compared with research from others, which revealed maximum percentage Removal of TPH using the same mass or dosage of bio stimulant, soil and crude oil sampled revealed 89.1%.From the findings of this research it has shown that the bio stimulant of vinegar stem possesses.The potential to be used in cleaning up of a polluted Sandy soil environment.