Extraction and Screening of Papain Enzyme from Papaya Leaves
Student: Sulaiman Salisu Ahmad (Project, 2025)
Department of Biochemistry
Northwest University, Kano, Kano State
Abstract
Microbial enzymes play a crucial role in various industrial and biotechnological applications. This study aimed to extract and analyze the enzymatic activity of a crude extract using spectrophotometric analysis and agar diffusion assay. The absorbance values at different substrate concentrations were used to generate a standard curve, from which the enzyme activity was determined to be 21.07 mg/ml. The qualitative screening of papain activity using the agar diffusion assay revealed significant enzymatic action, with the sample producing a halo diameter of 13 mm compared to 28 mm for the standard papain solution and 21 mm for the positive control. The negative control exhibited minimal activity with a halo diameter of 5 mm, confirming the enzymatic specificity of the extracted sample. These findings highlight the potential application of the extracted enzyme in industrial and pharmaceutical processes. Further optimization and purification are recommended to enhance enzyme yield and stability for commercial applications.
Keywords
For the full publication, please contact the author directly at: sulaimansalisuahmad3@gmail.com
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Institutions
- UMA UKPAI SCHOOL OF THEOLOGY, UYO, AKWA IBOM STATE (AFFL TO UNIVERSITY OF UYO) 1
- Umaru Ali Shinkafi Polytechnic, Sokoto, Sokoto State 24
- Umaru Musa Yaradua University, Katsina, Katsina State 28
- Umca, Ilorin (Affiliated To University of Ibadan), Kwara State 1
- University of Abuja, Abuja, Fct 117
- University of Africa, Toru-Orua, Bayelsa State 4
- University of Benin, Benin City, Edo State 362
- University of Calabar Teaching Hospital School of Health Information Mgt. 1
- University of Calabar, Calabar, Cross River State 240
- University of Ibadan, Ibadan, Oyo State 14