Isolation, Identification, and Characterization of Wound Associated Bacteria.

Student: FAVOUR ITUNUOLUWA ALAKE (Project, 2025)
Department of Microbiology
Bamidele Olumilua University of Edu. Science and Tech. Ikere Ekiti, Ekiti State

Abstract

‎Abstract ‎Wound infections are a major clinical concern due to the varying characteristics and antibiotic resistance patterns of the associated bacteria. The study collected and cultured wound samples from patients at the Ekiti State Teaching Hospital (EKSUTH) and identified isolates using Gram-staining and biochemical tests. ‎ ‎Key Findings: ‎ ‎Five probable bacterial species were identified: three Staphylococcus species, one Pseudomonas species (Y.M 2:4), and one Klebsiella species (A.M 2:1). ‎ ‎Pseudomonas spp. (Y.M 2:4) showed the highest resistance, with 80% resistance to gram-positive antibiotics and 0% to gram-negative antibiotics. ‎ ‎Klebsiella spp. (A.M 2:1) demonstrated moderate resistance (60% resistance to gram-negative antibiotics). ‎ ‎Some Staphylococcus spp. isolates showed minimal (0%) resistance. The results highlight the need for comprehensive microbiological characterization for effective wound treatment. ‎

Keywords
resistance wound species antibiotics characterization associated bacteria identified isolates staphylococcus
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