Prophylactic Potential of Myristica Leaves Extract Against Newcastle Disease Virus in Embryonated Chicken Eggs

Student: Ifechukwu Genevieve Chukwurah (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State

Abstract

The global cases of Newcastle disease among poultry farming in Nigeria has severely encroached the economic standard of poultry farmers. This study was undertaken in order to assess the activity of Myristica leaves extract against Newcastle Disease Virus (NDV). The phytochemical constituent of ethanolic extract of the plant was determined quantitatively using spectrophotometric and gravimetric methods. The activity of the extract and mixtures against NDV was determined using in vivo technique in embryonated chicken eggs. The photochemical constituents of the plant extract revealed the presence of alkaloids, tannins, flavonoids, phenolics, terpenoids, saponins and glycosides. There was decreased in egg weights from 63.18g to 44.12 and embryonic weights from 4.75g to 3.86g of the infected eggs after72h and these were significantly (P < 0.05) increased in these eggs protected with the extract. There was also significant (P< 0.05) reduction in infectivity of NDV as the embryo – egg weight ratio (EE) and neutralization values increased among the protected eggs. Therefore, Myristica leaves extract exhibited protective activity against NDV and could be used as alternative measure for preventing Newcastle diseases in poultry farming.

Keywords
prophylactic potential myristica leaves extract against newcastle disease virus embryonated
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