Isolation and Identification of Bacteria from Plastic Polluted Soil Using Isale Osun as Case Study, Osogbo, Osun State.

This study focuses on the isolation and identification of bacteria from microplastic- polluted soil, collected at the Isale-Osun dumpsite in Osogbo, Osun State, Nigeria. Microplastics, defined as plastic particles less than 5 mm in size, have accumulated widely in various environments, creating significant ecological and health challenges due to their durability and persistence. These particles act as vectors for pollutants and chemical additives, exacerbating environmental contamination. Soil samples from the dumpsite were subjected to microbiological analysis, targeting the isolation of bacteria capable of degrading plastics. The study utilized serial dilution and biochemical testing to identify bacterial species using microbiological standard. The microbiological assessment revealed a high prevalence of Bacillus subtilis, Bacillus licheniformis, and Streptococcus spp., known for their metabolic adaptability and plastic-degrading capabilities. Physicochemical analysis of the soil revealed alkaline conditions (pH 8.2 to 8.8) and moderate temperatures (25–29°C), both of which support microbial growth and enzymatic activities conducive to plastic degradation. Total viable bacterial counts ranged from 2.8 x 106 to 3.5 x 106 CFU/g, indicating substantial microbial presence. The findings suggest that microplastic-polluted soils host active microbial communities capable of contributing to plastic biodegradation. Bacillus species, in particular, demonstrate potential for bioremediation applications. This study recommends further research to enhance the efficacy of microbial degradation pathways and advocates for public awareness and policies addressing microplastic pollution. The study's insights highlight the role of microbial interventions in mitigating plastic waste, with implications for both environmental sustainability and public health.