Isolation and Antiobiotics Resistant Profile of Staphylococcus Aureus on Human Skin in Benue State University, Makudi.

Staphylococcus aureus expresses a variety of virulence factors that promote tissue adhesion and aid in the establishment of infection, tissue invasion and evasion from host immune response. S. aureus is a tough pathogen to treat due to its capacity to develop resistance to a variety of antibiotic classes. The aim of this study was to determine the Antibiotic resistance profile of Staphylococcus aureus isolated from the human skin. Sixty (60) hands of students from Benue State University were swabbed using swab sticks moistened with normal saline. The specimens were inoculated directly on Manitol Salt Agar (MSA) and incubated at 37°C for 18–24 hr. The isolates were sub-cultured on Mullen-Hinton agar. The inoculated plates were left at room temperature to dry for 3–5 minutes and a set of antibiotic discs. The result showed that, out of 28 isolates observed, 9 were susceptible to Erythromycin which represented 32.14%. 8(28.57%) were intermediate, while 11 representing 39.29% were observed to be resistant to the test antibiotics. Ten of the isolates were tested with Gentamycin out of which 9(90.00%) were susceptible and 1(10.00%) was resistant. The antibiotic susceptibility pattern of S. aureus to Amoxicillin showed that 10(35.71%) of the isolates were susceptible, 3(10.71%) were intermediate, while 15(53.57%) were observed to be resistant to the test antibiotic. The antibiotic susceptibility pattern of S. aureus to ceftriaxone showed that more isolates 17(60.71%) were resistant, followed by the number that was susceptible 6(21.43%) as well as the intermediate 1(3.57%). There was a significant relationship between the test antibiotics and the susceptibility ( ꭓ 2 = 18.246; df = 4; P = 0.001), intermediate reaction (ꭓ 2 = 9.730; df = 4; P = 0.045) and Resistance (ꭓ 2 = 33.313; df = 4; P = 0.000) of S. aureus isolates. This study reveals that S. aureus has gotten frighteningly resistant to many of common antimicrobials available, therefore it is recommended that people should avoid indiscriminate use of antibiotics.