Microbiological Assessment of Indoor and Outdoor Air of Microbiology Laboratory of Chukwuemeka Odumegwu Ojukwu University

This study was designed to assess the microbiological quality of indoor and outdoor air of Microbiology laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli Campus Anambra. A total of eight (8) air samples from the laboratory were collected and cultured on Nutrient Agar, MacConkey Agar, Nutrient Aga, Sabouraud Dextrose Agar and SalmonellaShigella Agar using sedimentation techniques. From this study, a total of twenty two (22) microbial species were isolated and identified from the indoor and outdoor air samples in some lecture halls and laboratory which belong to Bacillus sublitis, Staphylococcus aurues, Escherichia coli, Klebsilla spp, Streptococcus species, Aspergillus niger, Mucor alternaria and Aspergilus fumigatus etc. This finding is in agreement with the works by (Cox and Waters, 2005), who isolated three species each of Bacillus sublitis, Staphylococcus aurues, and Escherichia coli.The contamination rate and percentage distribution accessed on the Indoor laboratory Air samples in COOU revealed that among the indoor air isolates Micrococcus spp and Staphylococcus aureus are the most frequently occurring isolates with a high percentage occurrence of 2(7.4%) each, followed by Bacillus subtilis 4(14.8%), Cladosporium species, Penicillium species with the percentage of 2(7.4) and Klebsiella species 1(3.7%) at respectively, while the contamination rate and percentage distribution accessed on the outdoor laboratory Air samples in COOU revealed that among the outdoor air isolates Staphylococcus aureus, Micrococcus spp and Bacillus spp and are the most frequently occurring isolates with a high percentage occurrence of 3(10.7%) each, followed by Klebsiella species, Bacillus alvei and Cladosporium species, at 2(7.1%). Therefore, the present work shows that the outdoor laboratory air is heavily contaminated with bacteria and fungi loads than the indoor air sample