Upshot of Urine on Lipolytic Bacteria in the Soil

Student: Kosisochukwu Sandra Njoku (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State

Abstract

A good quality soil is characterized by adequate nutrients as a result of abundant nutrients cycling bacteria. Most soil in Nigeria has been subjected to different kinds of pollutants resulting from anthropogenic activities which have become a major threatening factor to the quality of soil. This study was carried out to evaluate the upshot of urine on lipolytic bacteria in the soil. A total of 30 composite soil samples were aseptically collected from urinating sites at Uli community using a sterile soil auger. The samples were analyzed for Total Heterotrophic Aerobic Bacteria (THABC) and Lipolytic Bacterial Count (LBC) using standard plate technique. The predominant bacterial isolates that aided nutrients cycling were appropriately characterized, and their densities in both impacted and non-impacted soil samples were enumerated. Also, the bacterial isolates were characterized based on macroscopy, microscopy, and biochemical characteristics. There was an increase in the THABC and LBC in the impacted soil, and Gram negative rods such as Pseudomonas and Klebsiella species and Micrococcus species (Gram positive) were the bacterial isolates. The study has revealed that urine in the soil increases microbial activities which invariably increases soil fertility.

Keywords
bacteria bacterial urine lipolytic characterized nutrients samples isolates upshot quality
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