Phytochemistry and Antibacterial Activity of Leaf Aqueous Extract of Diodia Sarmentosa Against Bacterial Isolates from Waste Dumpsite Within Futo Community

ABSTRACT Emerging cases of increase in microbial diseases infections caused by various pathogens has always been a matter of concern in the past and also at present. This has led to antibacterial resistance of existing conventional antibiotics by these pathogens. Antibacterial Resistance occurs when microbes shown no response to antimicrobial medicines, which has been declared top 10 global public health threat by WHO. Hence encourage the need to discover new antimicrobial agents from natural sources to combat drug resistant pathogens. Plant-derived compounds has proven to be effective as antimicrobial agents due to the presence of phytochemicals. Diodia sarmentosa is perennial medicinal plants which is known for its traditional use of treating ailments and disease. It contains certain properties including antioxidant, anti-inflammatory and antimicrobial. This study evaluates the phytochemical and antibacterial activity of Diodia sarmentosa against Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa and Enterococcus faecalis. Qualitative and quantitative phytochemical analysis, sensitivity test, Minimum Inhibitory Concentration and Minimum Bactericidal Concentration were carried out. Results obtained revealed that the bacterial isolates are sensitive to aqueous extract of plant material indicating potential antibacterial effectiveness. Also results obtained shows the MIC values, at 62.5mg/ml which is the minimum concentration at which bacteria are being inhibited. Additionally MBC values of Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa and Enterococcus faecalis are 500mg/ml, 250mg/ml, 125mg/ml and 500mg/ml respectively which indicates the concentration at which bacteria is killed completely. This study therefore shows that this product is effective against the four test organisms used. Furthermore, the research reveals the possible potential of Diodia sarmentosa as antimicrobial agents against these organisms in medical and possibly other applications.