Isolation and Identification of Microbial Diversity in Female Reproductive System

Student: Rukayat Oluwakemi Yayi (Project, 2025)
Department of Microbiology
Bamidele Olumilua University of Edu. Science and Tech. Ikere Ekiti, Ekiti State

Abstract

The unveiling of microbial diversity in female reproductive system plays a vital role in maintaining health, as imbalances may lead to infections or complications. This study focused on the isolation and identification of bacteria present in the vaginal region of volunteers to understand the composition of its microbial flora. The samples were promptly transported in sterile conditions to the microbiology laboratory and processed under controlled conditions to ensure microbial viability. The samples collected with swab stick were cultured on nutrient agar (NA) and potato dextrose agar (PDA). Bacteria colonies were observed and characterized based on morphology with biochemical tests, such as Gram staining, catalase, coagulase, oxidase, and indole assays were performed to identify the bacteria isolates. From our findings, morphological analysis revealed diverse colonies ranging from circular to irregular shapes with rough or smooth surfaces. Staphylococcus aureus was the only bacterium identified, which was the dominant microbes identified. Candida albicans. This study detected the presence of both commensal and opportunistic microorganisms in the female reproductive system, emphasizing the need for proper microbial balance to prevent infections.

Keywords
microbial female reproductive system bacteria isolation identification diversity infections samples
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