Effect of Crude Aqueous Stem Bark Extract of Khaya Senegalensis on Some Selected Gram-Positive Bacteria, Maiduguri Borno State by Hauwa Ahmad Ramat Hnd/ah/1114 a Project Submitted to the Department of Animal Health and Production Mohamet Lawan College of

Abstract This study investigated the antibacterial potential of crude aqueous stem bark extract of Khaya senegalensis against selected Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Enterococcus faecalis) at varying concentrations (400, 300, 200, and 100 mg/mL). Using the agar well diffusion method, the extract demonstrated concentration-dependent antibacterial activity, with the highest inhibition zones observed at 400 mg/mL (18.2±0.6 mm for S. aureus, 16.5±0.5 mm for B. subtilis, and 14.0±0.4 mm for E. faecalis). Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, terpenoids, and phenolic compounds, which are likely responsible for the observed antimicrobial effects. The results validate the traditional use of K. senegalensis in ethnomedicine for treating bacterial infections and suggest its potential as an alternative or complementary antimicrobial agent. However, the reduced efficacy at lower concentrations (100 mg/mL) indicates the need for standardized dosing. The study recommends further research to isolate active compounds, evaluate toxicity, assess synergistic effects with conventional antibiotics, and develop appropriate formulations. These findings contribute to the growing body of knowledge on plant-derived antimicrobials and highlight K. senegalensis as a promising candidate for developing natural antibacterial therapies, particularly in resource-limited settings where access to conventional antibiotics is challenging.