Effect of Acute Sleep Deprivation on Marker of Inflammation in the Stomach of Rat With Peptic Ulcer.

ABSTRACT
Acute sleep deprivation a condition and marked reduction of sleep duration over the short period. Sleep deprivation has many physiological effects, especially on a major factor in many health conditions as inflammation, which includes peptic ulcers. Unraveling acute sleep deprivation-induced changes regarding inflammatory markers in peptic ulcers in the stomach of rats. The condition that arises due to lack of sleep is pro-inflammatory cytokines at increased levels, such as tumor necrosis factor-alpha. These inflammatory levels significantly increase under conditions of sleep deprivation, and this has been observed in various animal models, including in rats. Acute sleep deprivation increases ulceration in acetic acid-induced ulcerations in rats when compared to those who experienced adequate sleep. Administration of acetic acid and sleep deprivation induces an additive effect in ulcer severity.
30 adult male Wistar rats (100-120g) were randomly divided into (Six) 6 groups consisting of 5 rats in each groups : Group 1: Normal Control Group 2: Peptic ulcer control , Group 3: Sleep deprivation day 3, Group 4: Peptic Sleep deprivation day 3 , Group 5: Peptic ulcer + Sleep deprivation day 3+1, Group 6:Peptic ulcer + Sleep deprivation day 3+1.
The wistar rats undergo acclimatization for 2 weeks in a plastic cage and they were fed only with water and pellet feed every day for the duration of 14 days.
The Second week half of the wistar rats were sedated and induced with a peptic ulcer with the use of (Acetic acid). After they have recovered from the sedation they were fed with pellet feed and 1 dual bearing sipper tube containing water. The rats stomach ulcer score and biochemical analysis were assessed on the 3th and 4th days . The Results was subjected to one way ANOVA analysis, presented as Mean ±SD and the values were considered significant at p=0.05
This study is done to investigate the impact of acute sleep deprivation on inflammatory markers in acetic acid-induced peptic ulcers in rats. Increased levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), were observed under sleep deprivation, exacerbating ulcer severity. The experiment was done to assess ulcer scores and biochemical analyses over several days. Results indicated significant variations in ulcer indices and protein levels across groups, highlighting the detrimental effects of sleep deprivation on inflammation and ulceration severity.