Fungi Isolated from Bioreactor Used for Bioremediation of Waste Water in Uli Anambra State

improvised bioreactor was developed using an empty bournvita container by creating an opening
for aeration, temperature and Ph assay. This is use to carry out bioremediation of wastewater with
a view for isolate the fungal organisms involved in the process. This research was carried out in
the microbiology laboratory of Chukwuemeka Odumegwu Ojukwu University Uli Anambra state,
This was done to identify the fungi associated with petroleum products polluted soil from Uli
Anambra state and wastewater from ogoni River state. The petroleum product polluted soil and
wastewater sample was added into the improvised bioreactor and stirred using the laboratory
handle brush which acts as the stirrer shaft. Bioaugumentation was instituted by addition of brain
heart infusion agar, yeast extract, agar, pepton water and addition of chloramphenicol
powder(antibiotics)optimization of operational parameters such as ph. temperature and aeration
and the integration of real time monitoring systems for process control.
SDA culture plate was prepared and inoculated using 2ml of the wastewater sample. The SDA
culture plate was incubated for 25-30°C and monitored regularly for fungal growth. The fungal
was cultivated using agar plate culture method for 7-14days.Resulis revealed that presence and
successful cultivation of the fungal within the bioreactor "ASPERGILLUS NIGER” species
CANDIDA SPECIES, there macroscopic and microscopic characteristics. Through systematic
experimentation and analysis, this project aims to achieve high pollution removal efficiencies
while maximizing the yield of aspergillus nigers. candida species biomass and to contribute
significantly to sustainable wastewater management practices by developing an innovative
bioreactor system that not only mitigate environmental pollution but also recovers valuable
microbial resources. The integration of wastewater remediation and microbial recovery presents a
dual benefit, promoting circular economy principles and advancing the field of environmental
biotechnology The recovered Aspergilus Niger and candida specie can be further utilized invarious
biotechnology applications, including enzyme production biofuel generation, and bio conversion
processes, thus contributing to sustainable waste water management and resource
recovery.