Phytochemical Screening and Anti Bacterial Activity of Commiphora Africana Against Clinical Isolate of Kliebsella and Pseudomonas Responsible for Deterioration of Infected Wound Using Two Different Solvent (aqueous and Ethanol)

ABSTRACT
Commiphora africana, a plant traditionally used in African folk medicine, was investigated for its phytochemical constituents and antibacterial activity against clinical isolates of Klebsiella and Pseudomonas responsible for wound infections.Some sample of Commiphora africana plant where collected from Gafasa Gezawa local government, using sterile polythene bag.between November and December,2024.Phytochemical screening revealed the presence of alkaloids, flavonoids, phenolics, and terpenoids in both aqueous and ethanol extracts. Wound infections caused by bacterial pathogens such as Klebsiella and Pseudomonas pose significant health risks due to their resistance to antibiotics. Commiphora africana, a medicinal plant widely used in traditional medicine, has been explored for its antibacterial potential. This study aimed to evaluate the phytochemical constituents and antibacterial efficacy of C. africana extracts prepared using aqueous and ethanol solvents against Klebsiella and Pseudomonas isolates from infected wounds. Standard phytochemical screening methods were employed to identify bioactive compounds, while the antibacterial activity was assessed using the agar well diffusion method. The phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins, saponins, and terpenoids in both extracts, with ethanol extract showing a higher concentration of these compounds. The antibacterial assay demonstrated that both extracts exhibited inhibitory effects against the test organisms, with the ethanol extract displaying significantly higher antibacterial activity compared to the aqueous extract. The findings suggest that C. africana possesses bioactive compounds with potential antibacterial properties, supporting its traditional use in wound healing. Further studies on the isolation and characterization of active compounds could contribute to the development of alternative antimicrobial agents.