Ameliorative Role of Kolaviron Against Aluminium Chloride-Induced Testicular Toxicity in Adult Male Mice

Exposure to aluminum chloride (AlCl₃), a widely used industrial compound, has been associated with oxidative stress and reproductive toxicity, particularly affecting testicular structure and function. This study investigates the potential protective effect of kolaviron, a bioflavonoid complex extracted from Garcinia kola, against AlCl₃-induced testicular damage in male mice. Thirty-four (34) adult male mice (of average weight 45g) were randomly assigned into eight groups of five animals each i.e group A - G: where group A: control, group B: tween-20, group C: AlCl₃-treated, group D: 100mg/kg kolaviron-treated, group E: 200mg/kg kolaviron-treated, group F:10mg/kg AlCl₃ followed by 100mg/kg kolaviron co-treated, group G: 10mg/kg AlCl₃ followed by 200mg kolaviron co-treated, the administration was given through oral gavage for 15 days. At the end of the experiment, the mice were sacrificed through cervical dislocation on the 16th day and the right testes were carefully harvested, fixed for immunohistological analysis, homogenized for biochemical analysis. The AlCl₃ group showed significant testicular toxicity, characterized by reduced germinal epithelium, potential maturation arrest, and hypotrophy, along with increased Malondialdehyde (MDA) levels. Body weight changes were statistically significant among groups, testicular weights did not show statistically significant differences. Testosterone levels showed significant differences between several groups. Kolaviron demonstrates significant ameliorative effects against aluminum chloride-induced testicular toxicity in adult male mice by mitigating oxidative stress and preserving testicular histoarchitecture. These findings suggest kolaviron's potential as a therapeutic agent for toxin-induced male infertility.
KEYWORDS: Aluminium chloride, Kolaviron, Testicular toxicity, Oxidative stress, Adult male mice.