Antioxidant Properties of Bryophyllum Pinnatum Leaves on Glut2 and It’s Dietary Implication on Lifespan of Drosophila melanogaster

Bryophyllum pinnatum is a medicinal plant with antioxidant, anti-inflammatory and antimicrobial properties that is used to promote wound healing, improve cardiovascular health, and manage diabetes. Its aim is to evaluate their potential in mitigating oxidative related damage. A library of phytochemicals from Bryophyllum pinnatum was compiled by extracting data from various databases, including PubChem and Google Scholar. These phytochemicals were then visualized using Discovery Studio software. A computational analysis of ligand interactions with GLUT2 was performed which revealed that several compounds exhibit strong binding affinities, with ergotamine showing the highest affinity .Mortality analysis indicated that 50 mg/kg B. pinnatum exerted protective effects, significantly reducing mortality relative to the control. However, exposure to 200 mg/kg accelerated mortality, implying cytotoxicity at high doses. Protein concentration declined significantly in the 50 mg/kg and 200 mg/kg groups (p < 0.05), suggesting altered metabolic activity. Nitric oxide levels decreased in a dose-dependent manner, with a substantial decline in the 50 mg/kg and 200 mg/kg groups, reflecting suppressed oxidative stress. Similarly, catalase activity was significantly lower in all treated groups, potentially due to compensatory antioxidant responses. In contrast, total thiols and glutathione transferase activity increased significantly across all B. pinnatum groups, signifying enhanced redox homeostasis. GLUT2 scavenging activity exhibited dynamic trends, where B. pinnatum outperformed quercetin at concentrations above 60 µg/mL, reinforcing its antioxidant potential. These findings suggest that B. pinnatum modulates oxidative stress and survival in D. melanogaster in a dose-dependent manner, warranting further exploration of its biological applications. Bryophyllum pinnatum is a plant with a rich phytochemical composition, including 32 bioactive compounds including flavonoids, alkaloids and phenolic acids.