Combined Antibacterial Activity of Ethanol Extracts of Psidium Guajava and Persea Americana Leaves on Mrsa

Methicillin Resistance Staphylococcus aureus strains have prompted this study to be able to opt in for an alternative. This study aims to evaluate the combined antibacterial effect of ethanol extracts of Persea americana and Psidium guajava leaves against Methicillin Resistance Staphylococcus aureus isolates. The powdered plant material was extracted using a cold maceration process using 80% v/v ethanol. Standard operating protocols were employed to analyze the phytochemical findings. The antibacterial sensitivity ofthe extracts was tested using the agar diffusion method. Ethanol extracts of Persea americana and Psidiumguajava were subjected to an antibacterial test separately and combined antibacterial effect against isolatesof Methicillin Resistant Staphylococcus aureus, (MRSA), by determining their zones of inhibition using the agar cup diffusion method. Results show varying zones of inhibition and minimum inhibitory concentration, MIC for Ethanol extracts for Persea americana and Psidium guajava leaves when testedagainst isolates of MRSA. Zones of inhibition ranging from 4-12mm and MIC ranged from 50mg/ml to200mg/ml, while that of ethanol extract of Psidium guajava zones of inhibition ranged from 4mm to 12mm,and minimum inhibitory concentration, MIC ranged from 12.5mg/ml to 200mg/ml. The combinedantibacterial effect of the ethanol extracts of Persea americana and Psidium guajava leaves revealed asynergistic effect with the zones of inhibition ranging from 20-30mm. In conclusion, the combinedantibacterial effect of both plants showed a synergistic antibacterial effect with very high antibacterial activity and this could be a good candidate to combat MRSA and also prevent drug resistance.