Screening of Amylase Producing Yeasts from Agricultural W Astes
Student: Ifunanya Immaculate Chilokwu (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of 3 waste samples including yam, plantain and mango peels were explored for isolateamylolytic yeast. The were washed, drained, diced, serially diluted and then cultured onyeast extract peptone dextrose agar supplemented with chloramphenicol. Sixmorphologically distinct colonies (YMI, YM3, PT2, MG3, MG1 and PT3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolyticactivity according to the qualitative test results with starch hydrolysis ratio of 3.46. Fiveyeast isolates did not show inhibition zones due to their inability to break down starch.YMI was identified as Geotrichum spp. The result of the work shows that amylase producing yeasts can be isolated from wastes (non-food materials) materials and can aspotential substrate for amylase production.
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Institutions
- Landmark University, Omu-Aran, Kwara State 1
- Lead City University, Ibadan, Oyo State 1
- Lens Polytechnic, offa, Kwara State. 214
- Madonna University, Elele, Rivers State 20
- Madonna University, Okija, Anambra State 2
- Mcpherson University, Seriki Sotayo, Ogun State 1
- Michael and Cecilia Ibru University, Owhrode, Delta State 1
- Michael Okpara University of Agriculture, Umudike 43
- Michael Otedola Col of Primary Educ. Epe, Lagos (affl To University of Ibadan) 8
- Modibbo Adama University, Yola, Adamawa State 15