Screening of Amylase Producing Yeasts from Agricultural Wastes
Student: Chukwunonso David Nwofor (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of 3 waste samples including yam, plantain and mango peels were explored for isolated amylolytic yeast. The were washed, drained, diced, serially diluted and then cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YMI, YM3, PT2, MG3, MG1 and PT3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity according to the qualitative test results with a starch hydrolysis ratio of 3.46. Fiveyeast isolates did not show inhibition zones due to their inability to break down starch.YMI was identified as Geotrichum spp. The result of the work shows that amylaseproducing yeasts can be isolated from wastes (non-food materials) materials and can as potential substrate for amylase production.
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For the full publication, please contact the author directly at: nwoforchukwunonso2@gmail.com
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- Precious Cornerstone University, Ibadan, Oyo State 1
- Prince Abubakar Audu University, Anyigba 30
- Redeemers University, Ede, Osun State 4
- Rhema University, Aba, Abia State 11
- Rivers State University of Science and Technology, Port Harcourt, Rivers State 3
- RIVERS STATE UNIVERSITY, PORT HARCOURT, RIVERS STATE 14
- Rufus Giwa Polytechnic, Owo, Ondo State 2
- Saadatu Rimi College of Edu, Kumbotso, Kano State (affiliated To Abu, Zaria) 1
- Salem University, Lokoja, Kogi State 4
- Samaru College of Agriculture (division of Agric Col, Abu) Zaria, Kaduna State 1