Screening of Amylase Producing Yeasts from Agricultural Wastes
Student: Chukwunonso David Nwofor (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of 3 waste samples including yam, plantain and mango peels were explored for isolated amylolytic yeast. The were washed, drained, diced, serially diluted and then cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YMI, YM3, PT2, MG3, MG1 and PT3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity according to the qualitative test results with a starch hydrolysis ratio of 3.46. Fiveyeast isolates did not show inhibition zones due to their inability to break down starch.YMI was identified as Geotrichum spp. The result of the work shows that amylaseproducing yeasts can be isolated from wastes (non-food materials) materials and can as potential substrate for amylase production.
Keywords
For the full publication, please contact the author directly at: nwoforchukwunonso2@gmail.com
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Institutions
- Al-Hikmah University, Ilorin, Kwara State 3
- AL-ISTIQAMAH UNIVERSITY, SUMAILA, KANO STATE 1
- Al-Qalam University, Katsina, Katsina State 5
- Alex Ekwueme Federal University, Ndufu-Alike, Ebonyi State 91
- Alvan Ikoku College of Education, Imo State, (Affl To Univ of Nigera, Nsukka) 11
- Ambrose Alli University, Ekpoma, Edo State 482
- Anambra State College of Health Technology, Obosi, Anambra State 1
- Auchi Polytechnic, Auchi, Edo State 516
- Auchi Polytechnic, Auchi, Edo State. (affl To Nnamdi Azikiwe University, Awka) 3
- Audu Bako College of Agriculture Danbatta, Kano, Kano State 54