In Vitro Evaluation of Anticoagulant Activity of Syzycium Aromaticum Cloves Aqueous Extract
Student: Shefiyat Abdulazeez (Project, 2025)
Department of Biochemistry
Federal Polytechnic, Nasarawa, Nasarawa State
Abstract
ABSTRACT The increasing demand for safer anticoagulant agents has led to the exploration of natural plant-based alternatives. This study evaluates the in vitro anticoagulant potential of aqueous extracts of Syzygium aromaticum (clove) through its ability to inhibit the extrinsic coagulation pathway, as measured by Prothrombin Time (PT). The extract yielded 44.2%, reflecting effective phytochemical recovery. Coagulation studies were performed using normal human plasma, with 3.2% sodium citrate serving as the positive control (PT = 100.00 ± 0.73 s) and 0.9% normal saline as the negative control (PT = 12.00 ± 0.57 s). The clove extract produced a concentration-dependent prolongation of PT: 45.5 ± 0.33 s (0.2 ml), 59.8 ± 0.52 s (0.3 ml), and 97.9 ± 0.23 s (0.5 ml). At the highest concentration, the PT closely approached that of the standard anticoagulant control. These results indicate that Syzygium aromaticum possesses significant anticoagulant properties, likely via inhibition of the extrinsic coagulation pathway. Further phytochemical and mechanistic studies are recommended to identify the bioactive compounds responsible.
Keywords
For the full publication, please contact the author directly at: Shefiyatabdulazeez2020@gmail.com
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- Federal University of Technology, Minna, Niger State 47
- Federal University of Technology, Owerri, Imo State 95
- Federal University Oye-Ekiti, Ekiti State 42
- Federal University, Birnin-Kebbi, Kebbi State 37
- Federal University, Dutse, Jigawa State 6
- Federal University, Dutsin-Ma, Katsina State 63
- Federal University, Gashua, Yobe State 3
- Federal University, Gusau, Zamfara State 14
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- Federal University, Lafia, Nasarawa State 6