In Vitro Evaluation of Anticoagulant Activity of Syzycium Aromaticum Cloves Aqueous Extract

Student: Shefiyat Abdulazeez (Project, 2025)
Department of Biochemistry
Federal Polytechnic, Nasarawa, Nasarawa State

Abstract

ABSTRACT The increasing demand for safer anticoagulant agents has led to the exploration of natural plant-based alternatives. This study evaluates the in vitro anticoagulant potential of aqueous extracts of Syzygium aromaticum (clove) through its ability to inhibit the extrinsic coagulation pathway, as measured by Prothrombin Time (PT). The extract yielded 44.2%, reflecting effective phytochemical recovery. Coagulation studies were performed using normal human plasma, with 3.2% sodium citrate serving as the positive control (PT = 100.00 ± 0.73 s) and 0.9% normal saline as the negative control (PT = 12.00 ± 0.57 s). The clove extract produced a concentration-dependent prolongation of PT: 45.5 ± 0.33 s (0.2 ml), 59.8 ± 0.52 s (0.3 ml), and 97.9 ± 0.23 s (0.5 ml). At the highest concentration, the PT closely approached that of the standard anticoagulant control. These results indicate that Syzygium aromaticum possesses significant anticoagulant properties, likely via inhibition of the extrinsic coagulation pathway. Further phytochemical and mechanistic studies are recommended to identify the bioactive compounds responsible.

Keywords
vitro evaluation anticoagulant activity syzycium aromaticum cloves aqueous extract
Filters
Institutions