Antibiotics Susceptibility Pattern of Bacteria Isolated from Shuttle Bus Door Handle Within Kaduna Polytechnic
Student: Obed Inalegwu Solomon (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
This study examined the bacterial contamination present on shuttle bus door handles within Kaduna Polytechnic and evaluated the antibiotic susceptibility patterns of the isolated organisms. A total of twenty (20) swab samples were collected from door handles of buses operating within the campus. Samples were cultured on nutrient agar and selective media to isolate and identify bacteria using standard microbiological techniques. The isolates obtained included Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Antibiotic susceptibility testing was performed using the Kirby–Bauer disc diffusion method on Mueller-Hinton agar. Results revealed that most isolates showed resistance to commonly used antibiotics such as ampicillin and tetracycline, while exhibiting higher sensitivity to ciprofloxacin and gentamicin. The findings highlight the public health risk associated with bacterial contamination on frequently touched surfaces and emphasize the need for regular sanitation and hand hygiene practices among shuttle users within the institution.
Keywords
For the full publication, please contact the author directly at: solomonobed148@gmail.com
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Institutions
- UMA UKPAI SCHOOL OF THEOLOGY, UYO, AKWA IBOM STATE (AFFL TO UNIVERSITY OF UYO) 1
- Umaru Ali Shinkafi Polytechnic, Sokoto, Sokoto State 24
- Umaru Musa Yaradua University, Katsina, Katsina State 28
- Umca, Ilorin (Affiliated To University of Ibadan), Kwara State 1
- University of Abuja, Abuja, Fct 117
- University of Africa, Toru-Orua, Bayelsa State 4
- University of Benin, Benin City, Edo State 362
- University of Calabar Teaching Hospital School of Health Information Mgt. 1
- University of Calabar, Calabar, Cross River State 240
- University of Ibadan, Ibadan, Oyo State 14