Antibiotics Susceptibility Pattern of Bacteria Isolated from Shuttle Bus Door Handle Within Kaduna Polytechnic
Student: Obed Inalegwu Solomon (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
This study examined the bacterial contamination present on shuttle bus door handles within Kaduna Polytechnic and evaluated the antibiotic susceptibility patterns of the isolated organisms. A total of twenty (20) swab samples were collected from door handles of buses operating within the campus. Samples were cultured on nutrient agar and selective media to isolate and identify bacteria using standard microbiological techniques. The isolates obtained included Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Antibiotic susceptibility testing was performed using the Kirby–Bauer disc diffusion method on Mueller-Hinton agar. Results revealed that most isolates showed resistance to commonly used antibiotics such as ampicillin and tetracycline, while exhibiting higher sensitivity to ciprofloxacin and gentamicin. The findings highlight the public health risk associated with bacterial contamination on frequently touched surfaces and emphasize the need for regular sanitation and hand hygiene practices among shuttle users within the institution.
Keywords
For the full publication, please contact the author directly at: solomonobed148@gmail.com
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Institutions
- Covenant Polytechnic, Aba, Abia State 1
- Covenant University, Canaan Land, Ota, Ogun State 4
- Crawford University of Apostolic Faith Mission Faith City, Igbesa, Ogun State 2
- Crescent University, Abeokuta, Ogun State 1
- Cross Rivers University of Technology, Calabar, Cross Rivers State 142
- Delta State Polytechnic, Ogwashi-Uku, Delta State 11
- Delta State Polytechnic, Otefe, Delta State 12
- Delta State University, Abraka, Delta State 139
- Ebonyi State University, Abakaliki, Ebonyi State 17
- Edo University, Iyamho, Edo State 10