Chemical Constituent and Insecticidal Activity of Essential Oil from Dried Unripe Citrus Reticulata
Student: Oluwadamilola Temitope Saka (Project, 2025)
Department of Chemistry
University of Ilorin, Kwara State
Abstract
Pulverized dried peels of Citrus reticulata (500g) were subjected to hydrodistillation for
four hours using a Clevenger-type apparatus. The resulting essential oil was analyzed
using Gas Chromatography-Mass Spectrometry (GC-MS), and its insecticidal properties
were evaluated through a contact toxicity bioassay against adult bean weevils
(Callosobruchus maculatus). The distillation process yielded 1.272% (w/w) of essential
oil. GC-MS analysis revealed that monoterpenoids constituted the major portion of the
oil, making up 99.6% of its composition. The dominant compounds identified D-
limonene (93.3%) and sabinene (2.1%). The oil demonstrated significant insecticidal
activity, causing mortality in the exposed insects. These findings suggest that the oil may
serve as a potential natural alternative to synthetic insecticides for controlling storage
pests.
Keywords
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Institutions
- Sokoto State University, Sokoto, Sokoto State 42
- St. Albert The Great Major Seminary, Abeokuta. (affl. To University of Benin) 1
- Sule Lamido University, Kafin Hausa, Jigawa State 4
- Tai Solarin University of Education, Ijagun, Ogun State 18
- Tansian University, Oba, Anambra State 1
- Taraba State University, Jalingo, Taraba State 32
- Temple-Gate Polytechnic, Osisioma, Abia State 1
- The Oke-Ogun Polytechnic, Saki, Oyo State 6
- The Polytechnic, Ibadan, Oyo State 13
- THOMAS ADEWUMI UNIVERSITY, OKO-IRESE, KWARA STATE 1