Optimization of Growth Conditions for in Vitro Propagation of Rosemary and the Occurrence of Microbial Contamination
Student: Rofiat Ifetayo (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
In vitro propagation of rosemary (Salvia rosmarinus) has gained increasing attention due to its medicinal, aromatic, and economic significance. However, optimizing growth conditions and preventing microbial contamination remain major constraints in tissue culture laboratories. This study focuses on evaluating the effects of culture media composition, plant growth regulators, and environmental conditions on shoot initiation and multiplication of rosemary. It also examines the frequency, sources, and types of microbial contamination encountered during culture establishment. Findings are expected to provide improved protocols for enhanced regenerative efficiency while minimizing contamination challenges. Ultimately, this research contributes to reliable mass propagation of rosemary for commercial and pharmaceutical applications.
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For the full publication, please contact the author directly at: ifetayorofiat8@gmail.com
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Institutions
- Abdul-Gusau Polytechnic, Talata-Mafara, Zamfara State 3
- Abia State Polytechnic, Aba, Abia State 24
- Abia State University, Uturu, Abia State 71
- Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State 3
- Abubakar Tafawa Balewa University, Bauchi, Bauchi State 15
- Abubakar Tatari Ali Polytechnic, Bauchi State. (affiliated To Atbu Bauchi) 1
- Achievers University, Owo, Ondo State 6
- Adamawa State University, Mubi, Adamawa State 8
- Adekunle Ajasin University, Akungba-Akoko, Ondo State 26
- Adeleke University, Ede, Osun State 1