Optimization of Growth Conditions for in Vitro Propagation of Rosemary and the Occurrence of Microbial Contamination
Student: Rofiat Ifetayo (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
In vitro propagation of rosemary (Salvia rosmarinus) has gained increasing attention due to its medicinal, aromatic, and economic significance. However, optimizing growth conditions and preventing microbial contamination remain major constraints in tissue culture laboratories. This study focuses on evaluating the effects of culture media composition, plant growth regulators, and environmental conditions on shoot initiation and multiplication of rosemary. It also examines the frequency, sources, and types of microbial contamination encountered during culture establishment. Findings are expected to provide improved protocols for enhanced regenerative efficiency while minimizing contamination challenges. Ultimately, this research contributes to reliable mass propagation of rosemary for commercial and pharmaceutical applications.
Keywords
For the full publication, please contact the author directly at: ifetayorofiat8@gmail.com
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Institutions
- HASSAN USMAN KATSINA POLYTECHNIC (NCE), KATSINA, KATSINA STATE 4
- Hassan Usman Katsina Polytechnic, Katsina, Katsina State 5
- Heritage Polytechnic, Ikot Udota, Akwa Ibom State 46
- Hussaini Adamu Federal Polytechnic, Kazaure, Jigawa State 8
- Ibrahim Badamasi Babangida University, Lapai, Niger State 24
- Igbinedion University, Okada, Benin City, Edo State 2
- Ignatius Ajuru University of Education, Port Harcourt, Rivers State 8
- Imo State Polytechnic, Umuagwo, Owerri, Imo State 2
- Imo State University, Owerri, Imo State 45
- Institute of Management and Technology, Enugu, Enugu State 11