Optimization of Growth Conditions for in Vitro Propagation of Rosemary and the Occurrence of Microbial Contamination
Student: Rofiat Ifetayo (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
In vitro propagation of rosemary (Salvia rosmarinus) has gained increasing attention due to its medicinal, aromatic, and economic significance. However, optimizing growth conditions and preventing microbial contamination remain major constraints in tissue culture laboratories. This study focuses on evaluating the effects of culture media composition, plant growth regulators, and environmental conditions on shoot initiation and multiplication of rosemary. It also examines the frequency, sources, and types of microbial contamination encountered during culture establishment. Findings are expected to provide improved protocols for enhanced regenerative efficiency while minimizing contamination challenges. Ultimately, this research contributes to reliable mass propagation of rosemary for commercial and pharmaceutical applications.
Keywords
For the full publication, please contact the author directly at: ifetayorofiat8@gmail.com
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- UMA UKPAI SCHOOL OF THEOLOGY, UYO, AKWA IBOM STATE (AFFL TO UNIVERSITY OF UYO) 1
- Umaru Ali Shinkafi Polytechnic, Sokoto, Sokoto State 24
- Umaru Musa Yaradua University, Katsina, Katsina State 28
- Umca, Ilorin (Affiliated To University of Ibadan), Kwara State 1
- University of Abuja, Abuja, Fct 116
- University of Africa, Toru-Orua, Bayelsa State 4
- University of Benin, Benin City, Edo State 362
- University of Calabar Teaching Hospital School of Health Information Mgt. 1
- University of Calabar, Calabar, Cross River State 239
- University of Ibadan, Ibadan, Oyo State 14