Optimization of Growth Conditions for in Vitro Propagation of Rosemary and the Occurrence of Microbial Contamination
Student: Rofiat Ifetayo (Project, 2025)
Department of Applied Biology
Kaduna Polytechnic, Kaduna
Abstract
In vitro propagation of rosemary (Salvia rosmarinus) has gained increasing attention due to its medicinal, aromatic, and economic significance. However, optimizing growth conditions and preventing microbial contamination remain major constraints in tissue culture laboratories. This study focuses on evaluating the effects of culture media composition, plant growth regulators, and environmental conditions on shoot initiation and multiplication of rosemary. It also examines the frequency, sources, and types of microbial contamination encountered during culture establishment. Findings are expected to provide improved protocols for enhanced regenerative efficiency while minimizing contamination challenges. Ultimately, this research contributes to reliable mass propagation of rosemary for commercial and pharmaceutical applications.
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For the full publication, please contact the author directly at: ifetayorofiat8@gmail.com
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Institutions
- Landmark University, Omu-Aran, Kwara State 1
- Lead City University, Ibadan, Oyo State 1
- Lens Polytechnic, offa, Kwara State. 214
- Madonna University, Elele, Rivers State 20
- Madonna University, Okija, Anambra State 2
- Mcpherson University, Seriki Sotayo, Ogun State 1
- Michael and Cecilia Ibru University, Owhrode, Delta State 1
- Michael Okpara University of Agriculture, Umudike 43
- Michael Otedola Col of Primary Educ. Epe, Lagos (affl To University of Ibadan) 8
- Modibbo Adama University, Yola, Adamawa State 15