Screening of Amylase Producing Yeast from Agricultural Waste
Student: Tochukwu Jemimah Francis (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of three waste samples, including yams, plantain, and mango peels, were explored for isolating amylolytic yeast. They were washed, drained, diced, and serially diluted before cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YM1, YM3, PT2, PT3, MG1, and MG3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity as indicated by the qualitative test results, with a starch hydrolysis ratio of 3.46. Five yeast isolates did not show inhibition zones due to their inability to break down starch. YM1 was identified as Geotrichum spp. The results of the work shows that amylase-producing yeasts can be isolated from wastes (non-food) materials and can be a potential substrate for amylase production.
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For the full publication, please contact the author directly at: francisjemimah96@gmail.com
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Institutions
- Abdul-Gusau Polytechnic, Talata-Mafara, Zamfara State 3
- Abia State Polytechnic, Aba, Abia State 24
- Abia State University, Uturu, Abia State 71
- Abraham Adesanya Polytechnic, Ijebu-Igbo, Ogun State 3
- Abubakar Tafawa Balewa University, Bauchi, Bauchi State 16
- Abubakar Tatari Ali Polytechnic, Bauchi State. (affiliated To Atbu Bauchi) 1
- Achievers University, Owo, Ondo State 6
- Adamawa State University, Mubi, Adamawa State 8
- Adekunle Ajasin University, Akungba-Akoko, Ondo State 27
- Adeleke University, Ede, Osun State 1