Screening of Amylase Producing Yeast from Agricultural Waste
Student: Tochukwu Jemimah Francis (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of three waste samples, including yams, plantain, and mango peels, were explored for isolating amylolytic yeast. They were washed, drained, diced, and serially diluted before cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YM1, YM3, PT2, PT3, MG1, and MG3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity as indicated by the qualitative test results, with a starch hydrolysis ratio of 3.46. Five yeast isolates did not show inhibition zones due to their inability to break down starch. YM1 was identified as Geotrichum spp. The results of the work shows that amylase-producing yeasts can be isolated from wastes (non-food) materials and can be a potential substrate for amylase production.
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Institutions
- Sokoto State University, Sokoto, Sokoto State 42
- St. Albert The Great Major Seminary, Abeokuta. (affl. To University of Benin) 1
- Sule Lamido University, Kafin Hausa, Jigawa State 4
- Tai Solarin University of Education, Ijagun, Ogun State 18
- Tansian University, Oba, Anambra State 1
- Taraba State University, Jalingo, Taraba State 32
- Temple-Gate Polytechnic, Osisioma, Abia State 1
- The Oke-Ogun Polytechnic, Saki, Oyo State 6
- The Polytechnic, Ibadan, Oyo State 13
- THOMAS ADEWUMI UNIVERSITY, OKO-IRESE, KWARA STATE 1