Screening of Amylase Producing Yeast from Agricultural Waste
Student: Tochukwu Jemimah Francis (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of three waste samples, including yams, plantain, and mango peels, were explored for isolating amylolytic yeast. They were washed, drained, diced, and serially diluted before cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YM1, YM3, PT2, PT3, MG1, and MG3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity as indicated by the qualitative test results, with a starch hydrolysis ratio of 3.46. Five yeast isolates did not show inhibition zones due to their inability to break down starch. YM1 was identified as Geotrichum spp. The results of the work shows that amylase-producing yeasts can be isolated from wastes (non-food) materials and can be a potential substrate for amylase production.
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For the full publication, please contact the author directly at: francisjemimah96@gmail.com
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- Covenant Polytechnic, Aba, Abia State 1
- Covenant University, Canaan Land, Ota, Ogun State 4
- Crawford University of Apostolic Faith Mission Faith City, Igbesa, Ogun State 2
- Crescent University, Abeokuta, Ogun State 1
- Cross Rivers University of Technology, Calabar, Cross Rivers State 142
- Delta State Polytechnic, Ogwashi-Uku, Delta State 11
- Delta State Polytechnic, Otefe, Delta State 13
- Delta State University, Abraka, Delta State 139
- Ebonyi State University, Abakaliki, Ebonyi State 17
- Edo University, Iyamho, Edo State 10