Screening of Amylase Producing Yeast from Agricultural Waste
Student: Tochukwu Jemimah Francis (Project, 2025)
Department of Microbiology
Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State
Abstract
Amylases are glycoside hydrolases, which are enzymes that facilitate the hydrolysis of complex sugars, such as starch and glycogen, into smaller sugar molecules. A total of three waste samples, including yams, plantain, and mango peels, were explored for isolating amylolytic yeast. They were washed, drained, diced, and serially diluted before cultured on yeast extract peptone dextrose agar supplemented with chloramphenicol. Six morphologically distinct colonies (YM1, YM3, PT2, PT3, MG1, and MG3) were picked and screened for their amylase production ability. Only YM1 exhibited amylolytic activity as indicated by the qualitative test results, with a starch hydrolysis ratio of 3.46. Five yeast isolates did not show inhibition zones due to their inability to break down starch. YM1 was identified as Geotrichum spp. The results of the work shows that amylase-producing yeasts can be isolated from wastes (non-food) materials and can be a potential substrate for amylase production.
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For the full publication, please contact the author directly at: francisjemimah96@gmail.com
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- UMA UKPAI SCHOOL OF THEOLOGY, UYO, AKWA IBOM STATE (AFFL TO UNIVERSITY OF UYO) 1
- Umaru Ali Shinkafi Polytechnic, Sokoto, Sokoto State 24
- Umaru Musa Yaradua University, Katsina, Katsina State 28
- Umca, Ilorin (Affiliated To University of Ibadan), Kwara State 1
- University of Abuja, Abuja, Fct 117
- University of Africa, Toru-Orua, Bayelsa State 4
- University of Benin, Benin City, Edo State 363
- University of Calabar Teaching Hospital School of Health Information Mgt. 1
- University of Calabar, Calabar, Cross River State 240
- University of Ibadan, Ibadan, Oyo State 14