In Vitro Anticoagulant Effect of Ethanolic Leaf Extracts of Justicia Secunda (blood Root)

Student: Monsurat Ahuoyiza Abdulhameed (Project, 2025)
Department of Applied Biochemistry
Federal Polytechnic, Nasarawa, Nasarawa State

Abstract

This study evaluates the in vitro anticoagulant activity of ethanolic leaf extracts of Justicia secunda (commonly known as Blood root) using Prothrombin Time (PT) as a marker for coagulation inhibition. The plant extract yielded 53.4%, indicating a substantial presence of bioactive phytochemicals. Citrated human plasma was treated with varying extract concentrations (0.2 ml, 0.3 ml, and 0.5 ml) and compared to a positive control (3.2% sodium citrate; PT = 100.00 ± 0.73 sec) and a negative control (0.9% normal saline; PT = 12.00 ± 0.57 sec).

Keywords
vitro anticoagulant effect ethanolic extracts justicia secunda blood
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